Bacteria (Culture)

According to Decree 280-2024 published on February 28, 2024, in the Gazette officielle du Québec, the amendment to the Appendix 1 of the Regulation respecting occupational health and safety (RSST, RLRQ, c. S-2.1, r. 13) will come into force on March 14, 2024. These regulatory changes have not yet been incorporated into the current Substance Data Sheets of the Sampling Guide for Air Contaminants in the Workplace. An update is currently underway and will be available as soon as possible. Consequently, the information contained in the data sheets of substances modified or added in Decree 280-2024 is partially inaccurate or incomplete. Please consult our infoLABO (in French only) for more details. Please contact our client service: [email protected] for further information.

Bacteria (Culture)

French name in the regulation: Bactéries

Not in the regulation.

CNESST – Toxicological Index Service

Consult REPTOX file:

Analysis:

Bactéries viables et cultivables (dénombrement)

Method: IRSST - 368

Sampling protocol

Sampling device:
Sampling media required:

#IRSST	Description
930	Polycarbonate filter

Parameters:
Flow rate: 2 L/min
TWA sampling volume: 40 L
STEL sampling volume: -
Remarks: The recommended sampling volumes are between 20 and 40 liters.

Analytical protocol

Principle: Stereomicroscopy (SM)
Precision (%): 22
Remarks: For sampling an environment with concentrations above 10,000 CFU/m³ of air. Bacterial count.

Reference

Dénombrement des bactéries et moisissures cultivables de l'air prélevées sur filtre polycarbonate. Méthodes analytique, méthode 368, Montréal, IRSST, 2011, 8 pages.

Bactéries viables et cultivables (Identification)

Method: IRSST - 341

Sampling protocol

Sampling device:
Sampling media required:

#IRSST	Description
930	Polycarbonate filter

Parameters:
Flow rate: 2 L/min
TWA sampling volume: 40 L
STEL sampling volume: -
Remarks: The recommended sampling volumes are between 20 and 40 liters.

Analytical protocol

Principle: MALDI-ToF mass spectrometry, Sanger sequencing or biochemical analysis (MALDI/Sanger/Bio)
Remarks: For sampling an environment with concentrations above 10,000 CFU/m³ of air. Bacterial identification.

Reference

Identification de bactéries cultivables. Method 341, Montreal, IRSST, 2024, 14 pages.

Bactéries viables et cultivables (dénombrement)

Method: IRSST - 264

Sampling protocol

Sampling device:
Sampling media required:

#IRSST	Description
5560	Agar-TSA bacteria agar

Parameters:
Flow rate: 28 L/min
TWA sampling volume: 140 L
STEL sampling volume: -
Remarks: The recommended sampling volumes are between 56 and 140 liters.

Analytical protocol

Principle: Stereomicroscopy (SM)
Precision (%): 7,2
Remarks: For sampling an environment with concentrations below 10 000 CFU/m³ of air. Bacterial count.

Reference

Dénombrement des bactéries et moisissures cultivables prélevées à l'aide d'un impacteur de marque Andersen. Method 264, Montreal, IRSST, 2021, 7 pages.

Bactéries viables et cultivables (Identification)

Method: IRSST - 341

Sampling protocol

Sampling device:
Sampling media required:

#IRSST	Description
5560	Agar-TSA bacteria agar

Parameters:
Flow rate: 28 L/min
TWA sampling volume: 140 L
STEL sampling volume: -
Remarks: The recommended sampling volumes are between 56 and 140 liters. For surface or liquid process sampling, contact [email protected].

Analytical protocol

Principle: MALDI-ToF mass spectrometry, Sanger sequencing or biochemical analysis (MALDI/Sanger/Bio)
Remarks: For sampling an environment with concentrations below 10 000 CFU/m³ of air. Bacterial identification.

Reference

Identification de bactéries cultivables. Method 341, Montreal, IRSST, 2024, 14 pages.